Immunoprophylaxis of rabbits against Rhipicephalus haemaphysaloides ticks using immunoaffinity purified 35 kDa midgut antigen
نویسندگان
چکیده
A 35 kDa midgut polypeptide of rhipicephalus haemaphysaloides was isolated for the first time by immunoaffinity chromatography using immunoglobulin ligands from rabbits immunized with 35 kDa midgut polypeptide. An experimental immunization study was conducted on 12 New Zealand white rabbits using affinity purified 35 kDa midgut antigen (Aff RhGM 35 kDa Ag) with Freund’s adjuvant. The humoral immune response was evaluated by ELISA. After tick challenge, reduction in the number, mass and oviposition capacity of engorged females was observed in the tick population that had fed on immunized animals. The results indicated a high efficacy of 83.3%, demonstrating the efficiency of the immune response elicited by 35 kDa midgut antigen to control the tick rhipicephalus haemaphysaloides.
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